A ratiometry-induced successive reusable electrochemical aptasensing platform: Efficient monitoring of aflatoxin B1 in peanut

Currently, most reusable aptasensors rely on the reassembly of aptamers to regenerate sensing interface. However, it remains challenging achieve successive aptasensor reusability. Herein, for first time, we demonstrate that a ratiometric strategy endows electrochemical high reusability realize detection aflatoxin B1 (AFB1). The model interface used thionine (THI)-graphene nanocomposite and ferrocene (Fc)-labeled aptamer output current signals (ITHI IFc). For analysis, specific recognition AFB1 by caused strip from electrode, value ITHI/IFc varied linearly with concentration over 0.01−100 ng mL−1 range. This relationship also worked when using single sensor multiple testing but completely failed ITHI or IFc alone. unique was ascribed capable eliminating environmental influence both signals. feasibility proposed validated applying AFB1, which showed excellent accuracy reliability compared official method, i.e., standard high-performance liquid chromatography-fluorescence (HPLC-FL). aptasensing platform can be universal advanced promotes practical applications particularly in field rapid assays.